Yes. ActionTrail limits the number of trails per account based on trail type:
Single-account trails: up to 5 per region.
Multi-account trail: 1 across all regions.
Trail for the Inner-ActionTrail feature: 1 across all regions.
These limits apply separately to each trail type. Choose a trail type based on your auditing scope and organizational structure.
References
For details on the differences between trail types, see Differences among a single-account trail, a multi-account trail, and a trail for the Inner-ActionTrail feature.